Efè diferan pati ekstrè Cistanche Deserticola sou efè ipoglisemi Db / Db sourit nwa Ⅲ

3 Rezilta eksperimantal

3.1 Rezilta detèminasyon kontni diferan pati ekstrè Cistanche deserticola/Cistanche deserticola

Mezire selon kondisyon yo anba "2.3", rezilta detèminasyon kontni nan diferan pati ekstrè nan Cistanche deserticola akTartschula deserticolayo montre nan Tablo 1.

NATIRÈL ÒGANIC ÒGANIC CISTANCHE AK 30% ECHINACOSIDE AK 12% ACTEOSIDE

3.2 Rezilta mezi endèks farmakolojik yo

3.2.1 Obsèvasyon jeneral

Peryòd eksperyans bèt la te 4 semèn, epi pwa kò sourit nwa yo te mezire chak semèn. Rezilta yo montre nan Tablo 2. Depi nan konmansman an rive nan fen eksperyans nan gavage, pwa sourit nwa yo nan gwoup modèl la kontinye ap ogmante, ki te konsistan avèk karakteristik pran pwa nan modèl dyabèt tip 2. Pwa rat nan gwoup kontwòl nòmal la te rete fondamantalman san chanjman. Apre 1 semèn nan administrasyon entragastric nan sourit nwa db/db nan diferan gwoup administrasyon, konpare ak gwoup modèl la, pran pwa nan rat nan chak gwoup administrasyon te amelyore nan divès degre, nan mitan ki efè a amelyorasyon nan glikozid total nan tartrum te la. pi gran. Siyifikativman (P<0.05); after 2 weeks and 4 weeks of intragastric administration of db/db black mice in different administration groups, the body weight of Amaranth oligosaccharide improved significantly (P<0.05 or P<0.01) . The above results show that different extracted parts of Cistanche deserticola and Branco deserticola have the effect of reducing the body weight of db / db black mice. The results are shown in Table 2.

3.2.2 Efè diferan pati ekstrè Cistanche deserticola sou nivo glikoz nan sourit nwa db/db

Yo te kenbe sik nan san sourit nwa db/db nan gwoup kontwòl nòmal la nan yon nivo nòmal (4.3 ~ 5.7 mmol·L-1), pandan y ap sik nan san sourit nwa yo nan gwoup modèl la te kontinye ogmante. (P<0.01). Compared with the model group, after 1 week of administration, the total polysaccharides, total oligosaccharides, and total glycosides of Cistanche deserticola group and the total polysaccharides, total oligosaccharides, and total glycosides FBG of Tartistan camphora group were reduced (P<0.01); after 2 weeks of administration, After 3 weeks of administration, the total glycosides FBG of tartschistanches were reduced (P＜0.05); after 3 weeks of administration, the total polysaccharides and total oligosaccharides FBG of tartans were reduced (P＜0.05 or P＜0.01); After taking the medicine for 4 weeks, the total polysaccharides, total oligosaccharides of Cistanche deserticola, and the total polysaccharides, total oligosaccharides, and total glycosides FBG of Cistanche deserticola were reduced (P <0.05). The results are shown in Table 3.

Nan tès tolerans glikoz oral la (gade Tablo 4), nivo mwayèn glikoz nan san gwoup kontwòl nòmal sourit nwa yo te rive nan yon pik nan 30 minit, ak Lè sa a, tonbe rapidman nan nivo nòmal yo. Sepandan, nivo mwayèn glikoz nan san sourit nwa yo nan gwoup modèl la kontinye rete nan yon nivo segondè apre yo fin rive nan pik la nan 30 minit. Konpare ak gwoup modèl la, glikoz nan san sourit nwa yo nan chak gwoup administrasyon te montre yon tandans desann nan divès degre apre yo te rive nan valè ki pi wo a, nan mitan ki total de polisakarid la, total oligosakarid ak total glikozid gwoup Tartschalna campestris gwoup la te refize pi vit. Anplis de sa, dapre kalkile AUC nan chak gwoup, rezilta yo te montre ke AUC nan sourit nwa nan chak gwoup administrasyon te pi ba pase sa yo ki nan gwoup modèl la, ak total polisakarid yo nan Cistanche deserticola ak glikozid yo total nan Tartistan deserticola redwi a. AUC sourit nwa yo nan 2 pwen tan (90 ak 120 min) apre administrasyon glikoz.Valè glikoz nan san(P<0.05 or P<0.01), total oligosaccharides of Cistanche deserticola and total polysaccharides of Tartschalisredwi glikoz nan sanvalè apre administrasyon glikoz pou 120 minit (P<0.05), total oligosaccharides of Tartschalis. Itredwi twa pwen tan yo(0, 90, ak 120 min) apre administrasyon glikoz ak zòn ki anba koub la (P<0.05 or P<0.01). The results showed that Cistanche deserticola and tartaric acid deserticola could improve the OGTT ability of db / db black mice.

Konpare ak gwoup kontwòl nòmal la, laendèks rezistans ensilin(HOMA-IR) akNivo HbA1c nan sourit nwa yonan gwoup modèl la ogmante (P<0.05), and the pancreatic islet β cell secretion index (HOMA-β) level decreased. Compared with the model group, the serum HbA1c content of the Cistanche deserticola oligosaccharide group, Cistanche deserticola polysaccharide group, Tartschistanche deserticola oligosaccharide group and Tartschistanche deserticola total glycosides group was significantly reduced (P <0.05); , the HOMA-IR index of the alcoholic acid agaricus oligosaccharide group and the alcoholic acid agaricus total glycosides group was significantly reduced (P <0.05); after 4 weeks of intervention in the administration group, compared with the model group, the black mice in each administration group The secretion index levels of pancreatic β-cells increased to varying degrees. The results are shown in Table 5.

Ensilin se yon òmòn pwoteyin ki se sitou sentèz ak sekrete pa selil ilo nan tisi pankreyas. Li patisipe nan metabolis glikoz kò a epi kenbe balans sik nan san kò a. Nivo INS serik yo ka reflete efè tretman dyabèt la. Li ka wè nan tablo 5 ke konpare ak gwoup nòmal la, nivo INS sourit nwa yo nan gwoup modèl la te siyifikativman redwi (P<0.01). After 4 weeks of intervention in the administration group, compared with the model group, the fasting serum insulin levels of black mice in each administration group increased to varying degrees, among which the total glycosides of Cistanche deserticola and Tartscholaris had the most obvious increase. The results showed that each administration group could increase the serum insulin level of db/db black mice and promote the secretion of insulin by pancreatic β-cells.

3.2.3 Efè diferan pati ekstrè Cistanche deserticola/Cistanche deserticola sou nivo estrès oksidatif fwa sourit nwa db/db

Nivo MDA nan fwa sourit nwa yo nan gwoup modèl la te siyifikativman pi wo pase sa nan gwoup nòmal la (P<0.05). After 4 weeks of intervention in the medication group, compared with the model group, the MDA levels of each medication group decreased to varying degrees (P < 0.05), among which Cistanche deserticola total polysaccharides, Cistanche deserticola total oligosaccharides, Cistanche deserticola total glycosides, and wine The level of MDA in the liver tissue of black mice treated with total glycosides of Cistanche deserticola and total oligosaccharides of Cistanche deserticola showed the most obvious decrease (P<0.05 or P<0.01). The results showed that Cistanche deserticola had a certain effect on reducing the MDA level in the liver tissue of db/db black mice, and the improvement effect was better than that of the alcoholic acid deserticola administration group.

Aktivite SOD nan tisi fwa sourit nwa yo nan gwoup modèl la te pi ba pase sa nan gwoup kontwòl nòmal la (P<0.05). When different extract parts of Cistanche deserticola and Tartscholaris are administered orally, the SOD activity in the liver tissue of db / db black mice can be increased. Compared with the model group, each administration group can restore SOD activity, among which the total glycosides of Cistanche deserticola group are different. There is statistical significance (P<0.05). The results are shown in Table 6.

3.2.4 Efè diferan pati ekstrè Cistanche deserticola/Cistanche deserticola sou metabolis grès epatik sourit nwa db/db

Yo nan lòd yo evalye efè a nan Cistanche deserticola / Tartrol deserticola sou nivo metabolis lipid serik sourit nwa db / db, makè ki gen rapò ak metabolis lipid dyabèt yo te detekte nan eksperyans la. Li ka wè nan tablo 7 ke konpare ak gwoup kontwòl nòmal la, nivo serik LDL-C nan gwoup modèl la te siyifikativman ogmante (P<0.05), while the HDL-C level was decreased. After 4 weeks of drug intervention, compared with the model group, the serum TG, TC and LDL-C levels of the Cistanche deserticola total polysaccharide, total oligosaccharide and total glycosides group and the Tartistan deserticola total polysaccharide, total oligosaccharide and total glycosides group were significantly lower than those of the model group. The levels of HDL-C decreased to varying degrees and the levels of HDL-C increased, among which the Cistanche deserticola total glycosides group had the effect of improving TC levels (P<0.05). The results showed that Cistanche deserticola and tartaric acid deserticola could improve the lipid metabolism disorder of db / db black mice to a certain extent.

3.2.5 Efè diferan pati ekstrè Cistanche deserticola/Cistanche deserticola sou UA, Cr ak mALB nan plasma ak pipi sourit nwa db/db

Apre 4 semèn nan administrasyon an, konpare ak gwoup modèl la, nivo UA, Cr ak mALB nan polisakarid total Cistanche deserticola, oligosakarid total ak gwoup glikozid total alkòl nan Cistanche deserticola te redwi a diferan degre. Anplis de diminye Cr, UA ak mALB nan pipi (P＜0.05 oswa P＜0.01), gwoup polisakarid agaricus asid alkòl la amelyore nivo UA, Cr ak mALB nan pipi (P<0.01). 05 oswa P<0.01), ethanol can improve the UA level in plasma (P<0.05), and the total glycosides of Cistanche canredwi plasma UA a, Cr ak mALB nivo sourit nwa db/db nan yon sèten limit.

3.2.6 Efè diferan pwodwi trete ak pati ekstrè Cistanche deserticola sou istopatoloji pankreyas ak ren sourit nwa db/db

Pa te gen okenn domaj patolojik nan tisi pankreyas sourit nwa yo nan gwoup kontwòl nòmal la. Bor klè nan ilo yo te vizib. Selil ilo yo te oval epi yo te ranje lòd nan sitoplasm la. Nwayo yo te wonn ak ilo yo te plis anpil ak pi gwo nan gwosè. Tisi pankreyas sourit nwa yo nan gwoup modèl la pa t gen ilo konplè. Bor yo nan ilo yo te klè ak fòm nan te enkonplè. Selil yo nan ilo yo te ranje nan yon fason dezòdone, ak yon ti kantite, atrofi, ak gwo domaj nan pankreyas la. Konpare ak rat yo nan gwoup modèl la, apre 4 semèn nan administrasyon entragastric nan diferan pati ekstraksyon nan Cistanche deserticola / Tartarchus deserticola, tisi a ilo pankreyas nan gwoup administrasyon an nan chak sit ekstraksyon pasyèlman refè, volim nan selil islet ogmante nan divès degre, ak selil ilo yo te gen limit klè. , aranjman an dezòdone amelyore. Pami yo, gwoup total glikozid Cistanche deserticola ak Tartschalne deserticola te montre pi bon rekiperasyon an.

gwoup kontwòl nòmal

Cistanche deserticola gwoup polisakarid

Tartschalna campestris gwoup polisakarid

gwoup modèl

Gwoup oligosakarid Cistanche deserticola

Gwoup oligosakarid tartracean

gwoup kontwòl pozitif

Cistanche deserticola total glikozid gwoup

Total glikozid gwoup Tarthola cistanche

Figi 3 Efè diferan pati ekstrè Cistanche deserticola/Cistanche deserticola sou patoloji pankreyas sourit nwa db/db (H&E, 200 ×)

Pa te gen okenn domaj evidan nan tisi ren sourit nwa yo nan gwoup kontwòl nòmal la. Estrikti glomerulèr la, fòm ak rapò ak kavite sist glomerulè a ​​te nòmal. Mi yo nan tib proximal ak distal yo te mens ak epè, ak gwosè a lumèn te nòmal. Estrikti, fòm, ak gwosè glomeruli sourit nwa yo nan gwoup modèl la te iregilye, epi glomeruli yo te atrofie ak ren yo.

Kavite balon an disparèt, tib proximal ak distal yo gen epesè miray inegal, diferan gwosè lumèn, limit klè, anfle ak nekwoz, ak yon gwo kantite enfiltrasyon selil enflamatwa. Konpare ak rat yo nan gwoup modèl la, apre 4 semèn nan administrasyon entragastric diferan pati ekstrè Cistanche deserticola / Tamarococcus deserticola, tisi ren gwoup administrasyon yo nan chak pati ekstrè amelyore, ak estrikti glomerulè, fòm, ak gwosè yo te fèmen. nan nòmal, ak tib proximal yo Miray la nan tubul distal la se mens, gwosè a nan lumèn nan amelyore, anfle a redwi, ak yon ti kantite enfiltrasyon selil enflamatwa ka toujou wè nan kèk pati. Pami yo, gwoup polisakarid Cistanche deserticola ak gwoup polisakarid deserticola alkòl te refè pi bon an.

3.2.7 Ekspresyon kolagen tip IV nan tisi ren db / db sourit nwa pa diferan pati ekstrè Cistanche deserticola / Litose deserticola sanble ak gwoup nòmal la.

Figi 4. Efè diferan pati extrait Cistanche deserticola/Tamarococcus deserticola sou patoloji ren sourit nwa db/db (H&E, 200 ×). Ekspresyon an nan pwoteyin kolagen tip-IV nan ren yo nan sourit nwa nan gwoup modèl la chanje anpil (P＜ 0.05). Konpare ak gwoup modèl la, mwayèn zòn matris manbràn ren sourit nwa yo nan chak gwoup administrasyon ogmante. Pami yo, ekspresyon kolagen tip-IV nan gwoup polisakarid Cistanche deserticola, gwoup oligosakarid tartschistanche deserticola, ak gwoup total glikozid tartschistanche deserticola te amelyore, pandan y ap ren pa te gen okenn diferans estatistik enpòtan nan ekspresyon kolagen tip IV. nan manbràn sousòl la.

gwoup kontwòl nòmal

Cistanche deserticola gwoup polisakarid

Cistanche deserticola gwoup polisakarid

gwoup modèl

Gwoup oligosakarid Cistanche deserticola

Gwoup oligosakarid Cistanche deserticola

gwoup kontwòl pozitif

Cistanche deserticola total glikozid gwoup

Cistanche deserticola total glikozid gwoup

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