Etid sou Fonksyon Neuroendocrin-Iminitè nan Cistanche Deserticola ak Diven Diven li yo vapè pwodwi nan modèl rat glucocorticoid-Ⅱ

3.2.4. Imunohistochimi

Apoptoz newòn kontwole pa anpil jèn apoptotik ak antiapoptotik, sitou jèn ki ankouraje apoptoz Bax ak jèn antiapoptotik fanmi jèn Bcl-2. Bcl-2 lokalize nan òganèl reyaktif ki pwodui oksijèn tankou mitokondri, retikul andoplasmik, ak manbràn nikleyè. Bcl-2 gen ti enpak sou radikal gratis oksijèn men li kapabanpeche domaj oksidatif nan selil yo. Bax transpòte soti nan sitoplasm nan manbràn mitokondriyo a, chanje estrikti manbràn mitokondriyo a, epi li ankouraje liberasyon sitokrom c ak endiksyon nan apoptoz.

NATIRÈL CISTANCHE TUBULOSA POU AMÉLIORE FONKSYON SEKSYÈL PHGS75% ECH 30% ACT 12%

Te gen yon ekspresyon nan sitoplasm adrenal Bax ak Bcl-2 pwoteyin. Tach la te montre tout patikil jòn. Konpare ak gwoup BC a, dansite optik mwayèn nan pwoteyin Bax nan gwoup MC a ogmante anpil (P< 0.01). Compared to that of the MC group, the average optical density in the CD-LD and WCD-LD groups decreased significantly (P < 0.01) (Figure 5(a)).

Figi 5 (b) montre ke konpare ak gwoup BC a, dansite optik mwayèn nan pwoteyin Bcl-2 nan gwoup MC a diminye anpil (P< 0.05). Compared to that of the MC group, the average optical density in the WCD-HD and CD-HD groups increased significantly (P < 0.01), while those of the WCD-MD and CD-MD groups increased (P < 0.05).

Pwoteyin caspase-3 lokalize sitou nan sitoplasm nan fòm orijinal li nan tisi nòmal epi li gen ekspresyon ki ba. Siyal pozitif yo jòn pal oswa jòn mawon. Konpare ak gwoup BC a, ekspresyon caspase-3 nan gwoup MC a ogmante anpil (P< 0.05). Compared to that of the MC group, the expression of caspase-3 in the WCD-HD and WCD-MD groups increased (P < 0.05) (Figure 5(c)).

Nan tisi nòmal yo, ekspresyon jèn Fas la te redwi, epi siyal pozitif pou ibridasyon in situ te jòn mawon. Jan yo montre nan Figi 5 (d), konpare ak gwoup BC a, dansite optik mwayèn nan pwoteyin Fas nan gwoup MC a ogmante (P< 0.05). Compared to that of the MC group, the average optical density in the WCD-HD and WCD-LD groups improved (P < 0.05) (Figure 5(d)).

Pwoteyin FasL la sitiye sitou nan manbràn selilè oswa sitoplasm, ak siyal pozitif li yo mawon. Konpare ak gwoup BC a, dansite optik mwayèn jèn FasL nan gwoup MC a ogmante anpil (P< 0.05). Compared to that of the MC group, the average optical density of the FasL gene in the WCD-HD, WCD-MD, CDHD, and CD-MD groups increased significantly (P < 0.01) (Figure 5(e)).

Rezilta yo te demontre ke CD ak WCD te kapabpwovoke apoptoz nan selil enflamatwaepi redwi domaj adrenal yo lè w ankouraje ekspresyon pwoteyin FasL la ak lye ak pwoteyin Fas la, aktive pwoteyin kaspas-3 epi deklanche yon kaspas kaspas. Efè gwoup WCD-HD te pi bon an.

3.3. Detèminasyon Fonksyon Iminitè

3.3.1. Endèks ògàn iminitè

Konpare ak sa yo nan gwoup BC a, koyefisyan timis la ak endèks larat gwoup MC a diminye anpil (P< 0.01). Compared to the MC group, the WCD-MD group had the greatest difference (P < 0.05). The thymus coefficient was almost back to the normal level. For the spleen index, the WCDMD, WCD-LD, and CD-MD groups showed significant differences compared with that of the MC group (P < 0.01) (see Figure 6). Therefore, WCD and CD treatment could protect the immune organs, and the effects of WCD were better.

NATIRÈL CISTANCHE TUBULOSA POU AMÉLIORE FONKSYON SEKSYÈL PHGS75% ECH 30% ACT 12%

3.3.2. Deteksyon sou-ansanm lenfosit T nan san periferik

Pousantaj selil CD4+ nan kadran anwo gòch la ak selil CD8+ nan kadran pi ba dwat nan BC a ak chak gwoup dwòg te pi wo pase sa ki nan gwoup MC la. Konpare ak gwoup BC a, rapò CD4+ThCD8+ diminye anpil nan gwoup MC (P< 0.01). Compared to that of the MC group, the ratio of CD4+ThCD8+ increased significantly in the PC group, the WCD group, and the CD-HD group (P < 0.01) (Figure 7).

3.3.3. Detèminasyon nivo IL-10, IL-6, IL{-2, TNF-Α, ak IFN-C nan serom

Figi 8 montre ke konpare ak gwoup BC a, nivo IL-6, TNF-, ak IFN-c ogmante anpil (P< 0.01). The level of IL-10 and IL-2 decreased in the MC group. Compared to that of the MC group, the level of IL-6, TNF-α, and IFN-c decreased and IL-10 and IL-2 increased in serum in each of the drug groups. The effect of WCD was better than the effect of CD, especially in the WCD-MD group, in which the effect was close to that of the PC group.

3.3.4. Rezilta Lymus Tissus Mikwoskòp Eksperyans Obsèvasyon

Figi 9 montre ke nan gwoup BC a, yon gwo kantite lenfosit te prezan. Lenfosit ak retikulosit yo te ranje san patipri nan sant la ak timosit wonn oswa oval gaye. Pa te gen okenn nekwoz, pa gen okenn enfiltrasyon selil enflamatwa, e pa gen okenn chanjman nòmal.

Gwoup la MC te montre yon gwo kantite tisi nekwoz lenfoyid, ak yon aranjman dezòdone nan lenfosit ak reticulocytes nan sant la. Timosit wonn oswa oval te disparèt, koripsyon selil masiv oswa nekwoz te evidan, e te gen enfiltrasyon selil enflamatwa ak chanjman patolojik nòmal.

Nan gwoup CD yo, karakteristik mòfolojik nan lenfosit yo te retounen nan nòmal, ak aranjman nan lenfosit santral ak retikulosit te prèske nòmal. Timosit wonn oswa oval te refè, men yon ti kantite lenfosit dejeneratif oswa nekrotik ak enfiltre selil enflamatwa rete.

Nan gwoup WCD yo ak gwoup PC a, karakteristik mòfolojik lenfosit yo te pi byen repare. Lenfosit yo ak retikulosit nan sant la te byen aliyen, timosit wonn oswa oval yo te prèske nòmal, men te rete yon ti kantite lenfosit enfiltre ak dejeneratif.

3.4. Ekspresyon jèn pwoteyin CaM

Valè RQ (CaM mRNA -actin mRNA) te konsidere kòm egzamine ekspresyon an nan mRNA CaM. RQ gwoup BC a te 1. Ekspresyon mRNA CaM nan ipotalamus nan gwoup MC a te ogmante anpil (P< 0.01), while the value decreased in the WCD-HD, WCD-MD, WCDLD, and CD-HD groups (P < 0.05) (Figure 10). The expression of CaM mRNA in the hypophysis in the MC group was lower than that in the BC group (P < 0.01). The expression in the WCD-HD, WCD-MD, WCD-LD, CD-HD, and CD-LD groups increased (P < 0.05), and the effect in the WCD groups was better than the effect in the CD groups.

4. Diskisyon

Farmakope Chinwa a [2] anrejistre ke CD ak WCD ka itilize nan klinik la kòm kalite prensipal yo nan moso dekokte. Apre yo fin vapè ak diven diri, laefè antiaging ak tonifyan ren-yangyo te amelyore. Atravè UPLC-QqQ-MS, nou detekte chanjman quantitative nan baz materyèl chimik pandan pwosesis la vapeur diven diri. Glikozid Iridoid yo te enstab ak kontni an nan glikozid sa yo diminye, menm vin detektab. Kontni an nan glikozid phenylethanol tou chanje, ki ta ka pwovoke chanjman nan farmakodinamik yo ak efè klinik la.

NATIRÈL CISTANCHE TUBULOSA POU AMÉLIORE FONKSYON SEKSYÈL PHGS75% ECH 30% ACT 12%

Modèl klasik kidney-yang defisyans la te repwodui pa piki lar nan kortikosteron [19]. Surdozaj kortikosteron ka anpeche sekrè CRH nan ipotalamus la. Pandan se tan, sekrè a nan ACTH te tou inibit. Nivo òmòn cortical adrenal la diminye, e konsa, fonksyon aks HPA te redwi. Ipofonksyon aks HPA se yon karakteristik komen nan defisi nan ren-yang [20]. Lè sa a, rat yo montre fenomèn "appauvrissement" ak sentòm yo an akò ak sa yo ki nan ren-yang deficiency nan klinik la.

Nan gwoup MC a, nivo CRH, ACTH, T, ak CORT nan serom rat diminye anpil (P< 0.01). After being treated by CD and WCD, the content of CRH, ACTH, T, CORT, and cortisol all increased to some degree, and the effect of WCD-MD was the best, almost equal to that in the PC group. These results indicated that the relative hormone balance in the HPA axis had been destroyed in the MC group, CD and WCD could improve the functional recovery of abnormal adrenal axis hormone levels, but the effect of WCD was better.

Modilasyon pwopòsyon manm fanmi Bcl-2 yo se youn nan mekanis prensipal yo kote FasThFasL medyatè chemen reseptè lanmò a, epi fanmi Bcl-2 kontwole chemen mitokondriyo a [21]. Faktè ki anpeche apoptoz Bcl-2 ak faktè ki pwovoke apoptoz, tankou Bax, tout fè pati fanmi Bcl-2 [22]. Apoptoz selil yo ka detèmine pa rapò relatif faktè ki anpeche apoptoz ak faktè ki pwovoke apoptoz [23]. Mitokondri yo gen fonksyon pòs santral pou plizyè fòm apoptoz [24]. Etid la te montre ke CD-MD ak WCD yo te kapab dedwi ekspresyon Bax ak ogmante Bcl-2 aamelyore deficiency nan ren-yang. Fas se pi gwo reseptè lanmò ki pwovoke apoptoz pa ligand ak ligand Fas (FasL) [25]. Ekspresyon Fas ak FasL nan gwoup MC a te ogmante, pandan y ap ekspresyon sa a te ogmante anpil nan gwoup WCD-MD (P).< 0.01) compared with the MC group. Both FasThFasL and BaxThBcl-2 systems transduce apoptotic signals to the caspase family [26]. Caspase-3 is one of the pivotal proteinases that initiate cell apoptosis. The activation of caspase-3 indicates that apoptosis enters an irreversible stage [27]. The expression of caspase-3 in the MC group increased and increased significantly in the WCD-HD and WCD-MD groups (P < 0.05).

Defisi nan fonksyon iminitè se youn nan manifestasyon enpòtan nan defisi nan ren-yang [28]. Nan etid sa a, nou te jwenn ke endèks larat la ak timis gwoup WCD-MD refè anpil (P< 0.01, P < 0.05). The immune response of the immune system is regulated mainly by the Th subgroups [29]. IL-2 is the growth factor for T cells, secreted by T cells and macrophages, participates in inflammatory regulation, regulates immunity, and increases infection resistance [30]. The level of IL-2 in the MC group decreased (P < 0.01), and after treatment with CD and WCD, the level of IL-2 recovered. IL-6 and TNF-α are proinflammatory cytokines. Increased levels of IL-6 and TNF-α cytokines cause cytokine imbalances and exacerbate inflammation. The levels of IL-6 and TNF-α increased in the MC group, while after treatment, the levels of IL-6 and TNF-α decreased. T cells are divided into CD4 cells and CD8 cells, and the CD4+ThCD8+ ratio can be used as an important index of immune regulation [31]. CD4+ThCD8+ in the MC group decreased (P < 0.01), while in the WCD and CDHD groups increased (P < 0.01).

Aks HPA a se sistèm regilasyon ki pi enpòtan paske li kontwole sekresyon glikokortikoyid ak sistèm andokrin lan pou kenbe fonksyon sekretè cortical adrenal la [32]. Ekspresyon twòp nan mRNA CaM nan tisi ipotalamus te gen rapò ak deficiency nan Yang ren. CD ak WCD ta ka bloke chanèl kalsyòm yo, anpeche ekspresyon mRNA CaM nan ipotalamus la, epi diminye aflu kalsyòm, kidonk diminye aktivite konplèks Ca2+·CaM.

Fòmasyon nan defisi nan ren-yang se yon pwosesis devlopman maladi kwonik. Ren se pa yon konsèp piman anatomik, epi li se yon tèm jeneral pou neuroendocrin-iminite ak sistèm jenitourinè. Rezo neroendokrin-iminitè (NEI) se yon sistèm regilasyon entegre enpòtan [33]. CD ak WCD ka kontwole fonksyon an nan aks HPAT ak amelyore rekiperasyon an nan deficiency nan ren-yang. Rezilta sa a bay baz eksperimantal pou prevansyon klinik ak tretman defisyans nan ren-yang.